NNi Learning Center

Below you will find resources for performing nanopore research and updates on solid-state nanopore technology.

Analysis example: double-stranded DNA

We walk you through a complete analysis workflow, using Nanolyzer to study translocation of dsDNA through a solid-state nanopore

Fitting events

Nanolyzer makes high-fidelity fitting of complex nanopore translocation events a simple process

Coming soon

What's next for Nanolyzer?

Visualizing your data

An introduction to visualization nanopore timeseries data using Nanolyzer

Clustering and substructure labeling

Clustering enables automatic and robust separation of complex data into groups for further analysis

Event database filtering

Learn how to separate nanopore translocation events into subpopulations using intuitive tools

Capture rate analysis

Capture rate is a tricky metric to analyze well in nanopore experiments. We present tools, tips, and tricks for getting it right

Visualizing event metadata

Nanolyzer enables beautiful nanopore experimental data visualizations with just a few clicks

Understanding your amplifier

Digital signal processing is a complex topic. We demystify some of the complexity in the context of nanopore measurements.

Characterizing a nanopore electrically

Most of the measurements you will do with a nanopore are electrical. We discuss how to extract useful information from these measurements.

Analyzing your data

Digital signal processing and data analysis is a daunting task. We present a tutorial using NNi Nanolyzer to simplify the process.

Piranha cleaning tutorial

Coming soon, a video tutorial on Piranha cleaning safety and best practices using NNi tools

Nanopore noise measurement tutorial

Coming soon, a tutorial demonstrating nanopore electrical noise measurement techniques and troubleshooting.

Solid-State Nanopore Tutorial

This tutorial is intended to quickly get researchers new to the field up to speed on the essentials of nanopore research.

NNi Nanopore Fabrication Software Tutorial

This video tutorial walks you through the basics of using the NNi nanopore fabrication software to make solid-state nanopores.

Nanopore Basics

A short review of nanopore basics giving some context to the rest of the tutorial posts and linking some essential references for new users.

How to detect biomolecules with a solid-state nanopore

We give some practical guidelines to assessing pore quality and optimizing experimental conditions for various biomolecular targets.

How to make a solid-state nanopore

Best practices to maximize your success making solid-state nanopores using NNi tools and the controlled breakdown method.

How to measure the size of your nanopore electrically

We provide some insight into the nuances of indirect nanopore size measurement, and tools to facilitate the process in your research.

NNi workflow tutorial

A video tutorial demonstrating the simplicity of use of NNi flow cells for rapid experimental setup.