The NNi nanopore fabrication software really does not require much of a tutorial. If you are using an NNi-supplied chip, all that really needs to happen is to select the corresponding protocol, pick your pore size, and press go. At that point you can just go get a coffee.
There are a few simple setup steps to go through, however. We have made a few video tutorials that go into more detail below.
If you need to use a membrane type that is not one of our standard suggestions, it is simple to change the protocol to accommodate, as shown in the video below. You can also refer to our recent publications [1,2] on the subject for general best practices in optimizing nanopore fabrication protocols. However, we strongly encourage you to reach out to us to discuss your membranes and how best to optimize a protocol for them, since exotic membrane types are often more expensive and we may be able to help avoid some of the common pitfalls encountered by novice breakdown users such as multiple pores.
 M. Waugh et al., “Solid-state nanopore fabrication by automated controlled breakdown,” Nat. Protoc., vol. 15, pp. 122–143, 2019, https://doi.org/10.1038/s41596-019-0255-2.
 C. Leung, K. Briggs, M.-P. Laberge, S. Peng, M. Waugh, and V. Tabard-Cossa, “Mechanisms of solid-state nanopore enlargement under electrical stress,” Nanotechnology, vol. 31, no. 44, p. 44LT01, 2020. https://doi.org/10.1088/1361-6528/aba86e
Last Updated: 2021-05-11